RT Journal Article
T1 Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients
A1 Martínez Román, Paula
A1 López Huertas, María Rosa
A1 Crespo Bermejo, Celia
A1 Arca Lafuente, Sonia
A1 Cortegano Jimeno, María Isabel
A1 Valle Millares, Daniel
A1 Gaspar, María Luisa
A1 Martín Carbonero, Luz
A1 Domínguez Domínguez, Lourdes
A1 Ryan Múrua, Pablo
A1 De los Santos, Ignacio
A1 De La Fuente-Moral, Sara
A1 Fernández Rodríguez, Amanda
A1 Coiras, Mayte
A1 Briz, Verónica
AB Coinfection with hepatitis C virus (HCV) influences HIV reservoir size. However, it is unknown whether this coinfection also induces a higher provirus transcription. Viral transcription is promoted by synergy between cellular factors such as NF-κB and the viral regulator Tat. The impact of HCV coinfection on HIV provirus transcription was analyzed in resting (r)CD4 T+ cells (CD3+CD4+CD25-CD69-HLADR-) and rCD4 T cells-depleted PBMCs (rCD4 T- PBMCs) from a multicenter cross-sectional study of 115 cART-treated HIV patients: 42 HIV+/HCV+ coinfected individuals (HIV+/HCV+), 34 HIV+ patients with HCV spontaneous clearance (HIV+/HCV−) and 39 HIV patients (HIV+). Viral transcription was assessed in total RNA through the quantification of unspliced, single spliced, and multiple spliced viral mRNAs by qPCR. Linear correlations between viral reservoir size and viral splicing were determined. A 3-fold increase of multiple spliced transcripts in rCD4 T+ cells of HIV+/HCV+ patients was found compared to HIV+ individuals (p < 0.05). As Tat is synthesized by multiple splicing, the levels of Tat were also quantified in these patients. Significant differences in single and multiple spliced transcripts were also observed in rCD4 T- PBMCs. Levels of multiple spliced mRNAs were increased in rCD4 T+ cells isolated from HIV+/HCV+ subjects, which could indicate a higher Tat activity in these cells despite their resting state.
PB MDPI
SN 2077-0383
YR 2020
FD 2020-07-03
LK https://hdl.handle.net/20.500.14352/8398
UL https://hdl.handle.net/20.500.14352/8398
LA eng
NO Martínez Román, P., López Huertas, M. R., Crespo Bermejo, C. et al. «Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients». Journal of Clinical Medicine, vol. 9, n.o 7, julio de 2020, p. 2091. DOI.org (Crossref), https://doi.org/10.3390/jcm9072091.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Instituto de Salud Carlos III
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
DS Docta Complutense
RD 18 mar 2026