RT Journal Article
T1 Class enzyme-based motors for “on the fly” enantiomer analysis of amino acids
T2 inglés
A1 García-Carmona, Laura
A1 Moreno Guzmán, María
A1 González, María Cristina
A1 Escarpa, Alberto
AB Here, two class-enzyme motors are properly designed allowing the rapid dispersion of the class-enzyme D-amino acid oxidase (DAO) and L-amino acid oxidase (LAO) for selective “on the fly” biodetection of D and L-amino acids (AAs), respectively. The efficient movement together with the continuous release of fresh class-enzyme leads to a greatly accelerated enzymatic reaction processes without the need of external stirring or chemical and physical attachment of the enzyme. Ultra-fast detection (<2 min) and accurate quantifications of L-phenylalanine (L-Phe) in plasma and whole-blood newborns samples diagnosed with Phenylketonuria and total D-AAs in Vibrio cholera cultures are pioneer illustrated as relevant examples of each enantiomer determination. These results opens clearly novel avenues in biosensing for fast screening diagnostics, decentralized monitoring and design of future points of care.
PB Elsevier
SN 0956-5663
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/93067
UL https://hdl.handle.net/20.500.14352/93067
LA eng
NO García-Carmona L, Moreno-Guzmán M, González MC, Escarpa A. Class enzyme-based motors for “on the fly” enantiomer analysis of amino acids. Biosensors and Bioelectronics 2017;96:275–80. https://doi.org/10.1016/j.bios.2017.04.051.
NO European Commission
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 11 abr 2025