RT Journal Article
T1 Enzyme Conformation Influences the Performance of Lipase‐powered Nanomotors
A1 Wang, Lei
A1 Marciello, Marzia
A1 Estévez‐Gay, Miquel
A1 Soto Rodríguez, Paul
A1 Luengo Morato, Yurena
A1 Iglesias‐Fernández, Javier
A1 Huang, Xin
A1 Osuna, Sílvia
A1 Filice, Marco
A1 Sánchez, Samuel
AB Enzyme-powered micro/nanomotors have myriads of potential applications in various areas. To efficiently reach those applications, it is necessary and critical to understand the fundamental aspects affecting the motion dynamics. Herein, we explored the impact of enzyme orientation on the performance of lipase-powered nanomotors by tuning the lipase immobilization strategies. The influence of the lipase orientation and lid conformation on substrate binding and catalysis was analyzed using molecular dynamics simulations. Besides, the motion performance indicates that the hydrophobic binding (via OTES) represents the best orienting strategy, providing 48.4 % and 95.4 % increase in diffusion coefficient compared to hydrophilic binding (via APTES) and Brownian motion (no fuel), respectively (with C[triacetin] of 100 mm). This work provides vital evidence for the importance of immobilization strategy and corresponding enzyme orientation for the catalytic activity and in turn, the motion performance of nanomotors, and is thus helpful to future applications.
SN 1433-7851
YR 2020
FD 2020-09-16
LK https://hdl.handle.net/20.500.14352/100718
UL https://hdl.handle.net/20.500.14352/100718
LA eng
NO Wang, Lei, et al. «Enzyme Conformation Influences the Performance of Lipase‐powered Nanomotors». Angewandte Chemie International Edition, vol. 59, n.o 47, noviembre de 2020, pp. 21080-87. DOI.org (Crossref), https://doi.org/10.1002/anie.202008339.
NO Fundación Nacional de Ciencias Naturales de China
NO Marie Sklodowska-Curie Fellowship
NO Special Funded Project of China Postdoctoral Science Foundation
NO Comunidad de Madrid
NO Federación Española de Enfermedades Raras
NO Ministerio de Economía, Comercio y Empresa (España)
NO Generalitat de Catalunya
NO European Research Council
NO Universidad Complutense de Madrid
NO Pro-CNIC Foundation
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Agencia Estatal de Investigación
DS Docta Complutense
RD 17 abr 2025