RT Journal Article
T1 “Super p53” Mice Display Retinal Astroglial Changes
A1 Salazar Corral, Juan José
A1 Gallego Pinazo, Roberto
A1 Hoz Montañana, María Rosa De
A1 Pinazo Durán, María Dolores
A1 Rojas López, María Blanca
A1 Ramírez Sebastián, Ana Isabel
A1 Serrano Espinosa, Manuel
A1 Ramírez Sebastián, José Manuel
AB Tumour-suppressor genes, such as the p53 gene, produce proteins that inhibit cell division under adverse conditions, as in the case of DNA damage, radiation, hypoxia, or oxidative stress (OS). The p53 gene can arrest proliferation and trigger death by apoptosis subsequent to several factors. In astrocytes, p53 promotes cell-cycle arrest and is involved in oxidative stress-mediated astrocyte cell death. Increasingly, astrocytic p53 is proving fundamental in orchestrating neurodegenerative disease pathogenesis. In terms of ocular disease, p53 may play a role in hypoxia due to ischaemia and may be involved in the retinal response to oxidative stress (OS). We studied the influence of the p53 gene in the structural and quantitative characteristics of astrocytes in the retina. Adult mice of the C57BL/6 strain (12 months old) were distributed into two groups: 1) mice with two extra copies of p53 (“super p53”; n = 6) and 2) wild-type p53 age-matched control, as the control group (WT; n = 6). Retinas from each group were immunohistochemically processed to locate the glial fibrillary acidic protein (GFAP). GFAP+ astrocytes were manually counted and the mean area occupied for one astrocyte was quantified. Retinal-astrocyte distribution followed established patterns; however, morphological changes were seen through the retinas in relation to p53 availability. The mean GFAP+ area occupied by one astrocyte in “super p53” eyes was significantly higher (p<0.05; Student’s t-test) than in the WT. In addition, astroglial density was significantly higher in the “super p53” retinas than in the WT ones, both in the whole-retina (p<0,01 Student’s t-test) and in the intermediate and peripheral concentric areas of the retina (p<0.05 Student’s t-test). This fact might improve the resistance of the retinal cells against OS and its downstream signalling pathways.
PB Public Library Science
SN 1932-6203
YR 2013
FD 2013-06-07
LK https://hdl.handle.net/20.500.14352/34755
UL https://hdl.handle.net/20.500.14352/34755
LA eng
NO Salazar Corral, J. J., Gallego Pinazo, r., Hoz Montañana, M. R. et al. «“Super P53” Mice Display Retinal Astroglial Changes». PLOS ONE, vol. 8, n.o 6, junio de 2013, p. e65446. PLoS Journals, https://doi.org/10.1371/journal.pone.0065446.
NO © 2013 Salazar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
NO Redes Temáticas de Investigación Cooperativa: Patologia Ocular del Envejecimiento, Calidad Visual y Calidad de Vida
NO Redes Temáticas de Investigación Cooperativa: Prevencion, deteccion precoz y tratamiento de la patologia ocular prevalente degenerativa y crónica
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO BSCH-UCM
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 11 abr 2025