RT Journal Article
T1 Novel Janus nanoparticles based on anisotropically-modified gold colloids for electrochemical aptasensor toward C-reactive protein
A1 Villalonga, Anabel
A1 Sánchez Sánchez, Alfredo
A1 Vilela García, Diana
A1 Mayol Hornero, Beatriz
A1 Martínez Ruiz, María Paloma
A1 Villalonga Santana, Reynaldo
AB Novel Janus nanoparticles based on Au colloids anisotropically modified with polyamidoamine dendrons were prepared though a masking/toposelective modification approach. These nanomaterials were further functionalized with horseradish peroxidase on the dendritic face and provided on the opposite metal surface with a ssDNA aptamer for C-reactive protein (CRP). The resulting nanoparticles were employed as biorecognition/signaling elements to construct an amperometric aptasensor with sandwich-type architecture for the specific detection of this cardiac biomarker. To do this, screen-printed carbon electrodes modified with electrodeposited Au nanoparticles and functionalized with anti-CRP aptamers were used as transduction interface. The aptasensor was employed for the amperometric detection of CRP (working potential: − 200 mV vs pseudo-Ag/AgCl) in the broad range from 10 pg·mL−1 to 1.0 ng·mL−1 with a detection limit of 3.1 pg·mL−1. This electroanalytical device also showed good specificity, reproducibility (RSD = 9.8%, n = 10), and stability and was useful to quantify CRP in reconstituted human serum samples, with a RSD of 13.3%.
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/129599
UL https://hdl.handle.net/20.500.14352/129599
LA eng
NO Villalonga, Anabel, et al. «Electrochemical Aptasensor Based on Anisotropically Modified (Janus-Type) Gold Nanoparticles for Determination of C-Reactive Protein». Microchimica Acta, vol. 189, n.o 8, agosto de 2022, p. 309. Springer Link, https://doi.org/10.1007/s00604-022-05420-4.
NO Spanish Ministry of Economy and Competitiveness
DS Docta Complutense
RD 25 feb 2026