RT Journal Article
T1 Urinary metabolomics study on the protective role of cocoa in Zucker diabetic rats via 1H-NMR-based approach
A1 Fernández Millán, Elisa
A1 Ramos, S
A1 Álvarez-Cilleros, David
A1 Samino, Sara
A1 Amigó, Nuria
A1 Correig, Xavier
A1 Chagoyen, Mónica
A1 Álvarez Escolá, Carmen
A1 Martí, María Ángeles
AB Cocoa constitutes one of the richest sources of dietary flavonoids with demonstrated anti-diabetic potential. However, the metabolic impact of cocoa intake in a diabetic context remains unexplored. In this study, metabolomics tools have been used to investigate the potential metabolic changes induced by cocoa in type 2 diabetes (T2D). To this end, male Zucker diabetic fatty rats were fed on standard (ZDF) or 10% cocoa-rich diet (ZDF-C) from week 10 to 20 of life. Cocoa supplementation clearly decreased serum glucose levels, improved glucose metabolism and produced significant changes in the urine metabolome of ZDF animals. Fourteen differential urinary metabolites were identified, with eight of them significantly modified by cocoa. An analysis of pathways revealed that butanoate metabolism and the synthesis and degradation of branched-chain amino acids andketone bodies are involved in the beneficial impact of cocoa on diabetes. Moreover, correlation analysis indicated major associations between some of these urine metabolites (mainly valine, leucine, and isoleucine) and body weight, glycemia, insulin sensitivity, and glycated hemoglobin levels.Overall, this untargeted metabolomics approach provides a clear metabolic fingerprint associated to chronic cocoa intake that can be used as a marker for the improvement of glucose homeostasis in a diabetic context.
PB MDPI
YR 2022
FD 2022-10-04
LK https://hdl.handle.net/20.500.14352/126057
UL https://hdl.handle.net/20.500.14352/126057
LA eng
NO Fernández-Millán, E.; Ramos, S.; Álvarez-Cilleros, D.; Samino, S.; Amigó, N.; Correig, X.; Chagoyen, M.; Álvarez, C.; Martín, M.Á. Urinary Metabolomics Study on the Protective Role of Cocoa in Zucker Diabetic Rats via 1H-NMR-Based Approach. Nutrients 2022, 14, 4127. https://doi.org/ 10.3390/nu14194127
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 30 mar 2026