RT Journal Article
T1 Dietary magnesium supplementation prevents and reverses vascular and soft tissue calcifications in uremic rats
A1 Díaz Tocados, Juan M.
A1 Peralta Ramírez, Alan
A1 Rodríguez Ortiz, María E.
A1 Raya, Ana I.
A1 López, Ignacio
A1 Pineda, Carmen
A1 Herencia, Carmen
A1 Montes de Oca, Addy
A1 Vergara, Noemí
A1 Steppan, Sonja
A1 Pendón Ruiz de Mier, M. Victoria
A1 Buendía, Paula
A1 Carmona, Andrés
A1 Carracedo Añón, Julia María
A1 Alcalá Díaz, Juan F.
A1 Frazao, Joao
A1 Martínez Moreno, Julio M.
A1 Canalejo, Antonio
A1 Felsenfeld, Arnold
A1 Rodríguez, Mariano
A1 Aguilera Tejero, Escolástico
A1 Almadén, Yolanda
A1 Muñoz Castañeda, Juan R.
AB Although magnesium has been shown to prevent vascular calcification in vitro, controlled in vivo studies in uremic animal models are limited. To determine whether dietary magnesium supplementation protects against the development of vascular calcification, 5/6 nephrectomized Wistar rats were fed diets with different magnesium content increasing from 0.1 to 1.1%. In one study we analyzed bone specimens from rats fed 0.1%, 0.3%, and 0.6% magnesium diets, and in another study we evaluated the effect of intraperitoneal magnesium on vascular calcification in 5/6 nephrectomized rats. The effects of magnesium on established vascular calcification were also evaluated in uremic rats fed on diets with either normal (0.1%) or moderately increased magnesium (0.6%) content. The increase in dietary magnesium resulted in a marked reduction in vascular calcification, together with improved mineral metabolism and renal function. Moderately elevated dietary magnesium (0.3%), but not high dietary magnesium (0.6%), improved bone homeostasis as compared to basal dietary magnesium (0.1%). Results of our study also suggested that the protective effect of magnesium on vascular calcification was not limited to its action as an intestinal phosphate binder since magnesium administered intraperitoneally also decreased vascular calcification. Oral magnesium supplementation also reduced blood pressure in uremic rats, and in vitro medium magnesium decreased BMP-2 and p65–NF-κB in TNF-α–treated human umbilical vein endothelial cells. Finally, in uremic rats with established vascular calcification, increasing dietary magnesium from 0.1% magnesium to 0.6% reduced the mortality rate from 52% to 28%, which was associated with reduced vascular calcification. Thus, increasing dietary magnesium reduced both vascular calcification and mortality in uremic rats.
PB Elsevier
SN 0085-2538
YR 2017
FD 2017-07
LK https://hdl.handle.net/20.500.14352/131833
UL https://hdl.handle.net/20.500.14352/131833
LA eng
NO Diaz-Tocados JM, Peralta-Ramirez A, Rodríguez-Ortiz ME, Raya AI, Lopez I, Pineda C, Herencia C, Montes De Oca A, Vergara N, Steppan S, Pendon-Ruiz De Mier MV, Buendía P, Carmona A, Carracedo J, Alcalá-Díaz JF, Frazao J, Martínez-Moreno JM, Canalejo A, Felsenfeld A, Rodriguez M, Aguilera-Tejero E, Almadén Y, Muñoz-Castañeda JR. Dietary magnesium supplementation prevents and reverses vascular and soft tissue calcifications in uremic rats. Kidney International 2017;92:1084–99. https://doi.org/10.1016/j.kint.2017.04.011.
NO Acknowledgments:This work has been partially supported by a Spanish government grant from the Programa Nacional I+D+I 2008-2011 from the Instituto de Salud Carlos III (PI14/00638 and PI14/00467) with cofinancing from European Funds (FEDER); Consejería de Economía, Innovación, Ciencia y Empleo from the Junta de Andalucía (CVI-7925); Fresenius Medical Care; EUTOX; and REDINREN from Instituto de Salud Carlos III.
NO European Commission
NO Instituto de Salud Carlos III (España)
NO Junta de Andalucía
NO Fresenius Medical Care
NO EUTOX
NO REDINREN
DS Docta Complutense
RD 27 feb 2026