RT Journal Article
T1 Magnetic Janus micromotors for fluorescence biosensing of tacrolimus in oral fluids
A1 Glahn Martínez, Ana Bettina
A1 Jurado-Sánchez, Beatriz
A1 Benito Peña, María Elena
A1 Escarpa, Alberto
A1 Moreno Bondi, María Cruz
AB Tacrolimus (FK506) is a macrolide lactone immunosuppressive drug that is commonly used in transplanted patients to avoid organ rejection. FK506 exhibits high inter- and intra-patient pharmacokinetic variability, making monitoring necessary for organ graft survival. This work describes the development of a novel bioassay for monitoring FK506. The bioassay is based on using polycaprolactone-based (PCL) magnetic Janus micromotors and a recombinant chimera receptor that incorporates the immunophilin tacrolimus binding protein 1A (FKBP1A) tagged with Emerald Green Fluorescent Protein (EmGFP). The approach relies on a fluorescence competitive bioassay between the drug and the micromotors decorated with a carboxylated FK506 toward the specific site of the fluorescent immunophilin. The proposed homogeneous assay could be performed in a single step without washing steps to separate the unbound receptor. The proposed approach fits the therapeutic requirements, showing a limit of detection of 0.8 ng/mL and a wide dynamic range of up to 90 ng/mL. Assay selectivity was evaluated by measuring the competitive inhibition curves with other immunosuppressive drugs usually co-administered with FK506. The magnetic propulsion mechanism allows for efficient operation in raw samples without damaging the biological binding receptor (FKBP1A-EmGFP). The enhanced target recognition and micromixing strategies hold considerable potential for FK506 monitoring in practical clinical use.
PB Elsevier
SN 0956-5663
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/93325
UL https://hdl.handle.net/20.500.14352/93325
LA eng
NO Glahn-Martínez, Bettina, et al. «Magnetic Janus Micromotors for Fluorescence Biosensing of Tacrolimus in Oral Fluids». Biosensors and Bioelectronics, vol. 244, enero de 2024, p. 115796. https://doi.org/10.1016/j.bios.2023.115796.
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 21 ago 2024