RT Journal Article
T1 Aggregatibacter actinomycetemcomitans Growth in Biofilm versus Planktonic State: Differential Expression of Proteins
A1 Llama Palacios, María Arantxazu
A1 Potupa, Oksana
A1 Sánchez Beltrán, María Del Carmen
A1 Figuero Ruiz, Elena
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB Aggregatibacter actinomycetemcomitans (Aa) is a pathogenic bacterium residing in the subgingival plaque biofilm strongly associated with the pathogenesis of periodontitis. The aim of this investigation was to study the protein differential expression of Aa when growing on biofilm compared with planktonic state using proteomic analysis by the 2D-DIGE system. Eighty-seven proteins were differentially expressed during biofilm growth (1.5-fold, p < 0.05), with 13 overexpressed and 37 down-expressed. Those repressed were mainly proteins involved in metabolism, biosynthesis, and transport. The overexpressed proteins were outer membrane proteins (OMPs) and highly immunogenic proteins such as YaeT (OMP), FtsZ, OMP39, OMP18/16, the chaperone GroEL, OMPA, adenylate kinase (Adk), and dihydrolipoamide acetyltransferase. The enrichment fractions of the OMPs from biofilm and planktonic states were obtained, and these proteins were analyzed by Western blotting with human serum from a periodontitis patient and one healthy control. These immunogenic proteins overexpressed in the biofilm may represent candidate virulence factors
PB ACS
SN 1535-3893
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/109195
UL https://hdl.handle.net/20.500.14352/109195
LA eng
NO Llama-Palacios A, Potupa O, Sánchez MC, Figuero E, Herrera D, Sanz M. Aggregatibacter actinomycetemcomitans Growth in Biofilm versus Planktonic State: Differential Expression of Proteins. J Proteome Res. 2017 Sep 1;16(9):3158-3167. doi: 10.1021/acs.jproteome.7b00127
NO Centro para el Desarrollo Tecnológico Industrial (CDTI, España)
DS Docta Complutense
RD 16 dic 2025