RT Journal Article
T1 MiRNA post-transcriptional modification dynamics in T cell activation
A1 Rodríguez-Galán, Ana
A1 Dosil, Sara
A1 Gómez, Manuel José
A1 Fernández-Delgado, Irene
A1 Fernández Messina, Lola María
A1 Sánchez-Cabo, Fátima
A1 Sánchez-Madrid, Francisco
AB T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modification kinetics in human primary CD4+ T cells upon T cell receptor (TCR) or type I interferon stimulation. This analysis identified differential expression of multiple miRNAs not previously linked to T cell activation. Remarkably, upregulated miRNAs showed a higher frequency of 3′ adenylation. TCR stimulation was followed by increased expression of RNA modifying enzymes and the RNA degrading enzymes Dis3L2 and Eri1. In the midst of this adverse environment, 3′ adenylation may serve a protective function that could be exploited to improve miRNA stability for T cell-targeted therapy.
PB Elsevier
SN 2589-0042
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/97230
UL https://hdl.handle.net/20.500.14352/97230
LA eng
NO Rodríguez-Galán, Ana, et al. «MiRNA Post-Transcriptional Modification Dynamics in T Cell Activation». iScience, vol. 24, n.o 6, junio de 2021, p. 102530. https://doi.org/10.1016/j.isci.2021.102530.
NO AcknowledgmentsWe thank Miguel Vicente-Manzanares and Simon Bartlett for help with English. We also thank the CNIC Genomics and Bioinformatics Units for technical support.This work was supported by grants SAF2017-82886-R from the Spanish Ministry of Economy and Competitiveness (MINECO), grant S2017/BMD-3671-INFLAMUNE-CM from the Comunidad de Madrid, a grant from the Ramón Areces Foundation “Ciencias de la Vida y la Salud” (XIX Concurso-2018) and a grant from Ayudas Fundación BBVA an Equipos de Investigación Científica (BIOMEDICINA-2018) and ““la Caixa”” Banking Foundation (HR17-00016). BIOIMID (PIE13/041) from Instituto de Salud Carlos III, CIBER Cardiovascular (CB16/11/00272, Fondo de Investigación Sanitaria del Instituto de Salud Carlos III and co-funding by Fondo Europeo de Desarrollo Regional FEDER). The Centro Nacional de Investigaciones Cardiovasculares (CNIC, Spain) is supported by the Ministerio de Economía y Competitividad-Spain and the Pro-CNIC Foundation.A.R.G., S.G.D., and I.F.D. were supported by the FPU program (Spanish Ministry of Education).
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
NO European Commission
NO Instituto de Salud Carlos III
NO Fundación BBVA
NO Fundación "la Caixa"
DS Docta Complutense
RD 8 abr 2025