%0 Journal Article
%A Castromil Benito, Estela S.
%A Betancor, Diana
%A Parrón Ballesteros, Jorge
%A Bueno Díaz, Cristina
%A Gutiérrez Díaz, Gloria
%A Turnay, Javier
%A de las Heras, Manuel
%A Cuesta Herranz, Javier
%A Villalba, Mayte
%A Pastor Vargas, Carlos
%T Walnut jug r 1 is responsible for primary sensitization among patients suffering walnut-hazelnut 2S albumin cross-reactivity
%D 2024
%U https://hdl.handle.net/20.500.14352/109761
%X Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed that 85% of patients recognized Jug r 1, walnut 2S albumin, which was associated with the development of severe symptoms; 50% of them corecognized hazelnut 2S albumin, Cor a 14. Both allergens were isolated using chromatographic techniques. Inhibition ELISAs revealed that Jug r 1 strongly inhibited the binding of Cor a 14-specific IgE, but Cor a 14 only partially inhibited Jug r 1-specific IgE binding. Our results showed that patients sensitized to walnut/hazelnut 2S albumins were not a homogeneous population. There were patients sensitized to specific epitopes of walnut 2S albumins and patients sensitized to cross-reactive epitopes between walnut and hazelnut, with Jug r 1 being the primary sensitizer.
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