RT Journal Article
T1 Walnut jug r 1 is responsible for primary sensitization among patients suffering walnut-hazelnut 2S albumin cross-reactivity
A1 Castromil Benito, Estela S.
A1 Betancor, Diana
A1 Parrón Ballesteros, Jorge
A1 Bueno Díaz, Cristina
A1 Gutiérrez Díaz, Gloria
A1 Turnay, Javier
A1 de las Heras, Manuel
A1 Cuesta Herranz, Javier
A1 Villalba, Mayte
A1 Pastor Vargas, Carlos
AB Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed that 85% of patients recognized Jug r 1, walnut 2S albumin, which was associated with the development of severe symptoms; 50% of them corecognized hazelnut 2S albumin, Cor a 14. Both allergens were isolated using chromatographic techniques. Inhibition ELISAs revealed that Jug r 1 strongly inhibited the binding of Cor a 14-specific IgE, but Cor a 14 only partially inhibited Jug r 1-specific IgE binding. Our results showed that patients sensitized to walnut/hazelnut 2S albumins were not a homogeneous population. There were patients sensitized to specific epitopes of walnut 2S albumins and patients sensitized to cross-reactive epitopes between walnut and hazelnut, with Jug r 1 being the primary sensitizer.
PB ACS Publications
YR 2024
FD 2024-07-31
LK https://hdl.handle.net/20.500.14352/109761
UL https://hdl.handle.net/20.500.14352/109761
LA eng
NO Castromil-Benito ES, Betancor D, Parrón-Ballesteros J, Bueno-Díaz C, Gutiérrez-Díaz G, Turnay J, Heras ML, Cuesta-Herranz J, Villalba M, Pastor-Vargas C. Walnut Jug r 1 is Responsible for Primary Sensitization among Patients Suffering Walnut-Hazelnut 2S Albumin Cross-Reactivity. J Agric Food Chem. 2024 Aug 14;72(32):18162-18170. doi: 10.1021/acs.jafc.4c03603
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025