%0 Generic
%A Bonnin Arias, Cristina Natalia
%A Chamorro Gutiérrez, E.
%A Urbano Rodríguez, R.
%A Sánchez Ramos, Celia
%T Protein and lipid cleaning of blue light-filtering contact lens with cleaning and lubricant drops.
%D 2012
%U https://hdl.handle.net/20.500.14352/108658
%X Purpose: To determine the efficacy of moisturizing Cleaning Drops ® (Avizor) in eliminating protein and lipid deposits over contact lenses with and without blue light filtering.Methods: Protein and lipid deposits were evaluated in non-blue light-filtering contact lens (Iotrafilcon B 33%WC) and blue light-filtering contact lens (Profilcon A 52%WC) after a cleaning process with moisturizing Cleaning Drops®. The control group consisted of untreated lenses. Analysis of lipid deposits was carried out by incubation of the contact lenses in a 1.5 ml solution composed of 0.17 mg/ml of free cholesterol and 0.23 mg/ml of cholesterol oleate for 96 hours. Cholesterol was extracted using a standard protocol and then, analyzed using High Performance Liquid Chromatography (HPLC) using a reverse phase C-18 Zorabax column (4.6×250 mm) (Phenomenex, Torrance, CA). The UV detector was set at 206 nm. Analysis of protein deposits was carried out by incubation of the contact lenses in a 1.5 ml solution composed of 0.5 mg/ml of albumin and 7.5μl/ml of 14C-albúmin for 96 hours. At the end of the specified incubation period, radioactive counts were determined in the lenses using liquid scintillation technique.Results/Data: For the blue light-filtering contact lens, in the control group study, an amount of 24.70±9.6μm/lens of free cholesterol, 19.94±8.82μm/lens of esterified cholesterol, 44.65±12.14μm/lens of total cholesterol and 2.96±0.23μm/lens of albumin was observed. Once the cleaning by moisturizing Cleaning Drops® was done, an amount of 8.96±0.62μm/lens of free cholesterol, 3.78±1.19μm/lens of esterified cholesterol, 12.74±2.83μm of total cholesterol and 1.17±0.23μm/lens of albumin was observed. For non-blue light-filtering contact lens in the control group study, an amount of 17.47±2.35μm/lens of free cholesterol, 7.50±1.51μm/lens of esterified cholesterol, 24.97±3.61μm/lens of total cholesterol and 1.73±0.54μm/lens of albumin was detected. Once the cleaning by moisturizing Cleaning Drops® was done, an amount of 3.02±0.06μm/lens of free cholesterol, 10.13±0.58μm/lens of esterified cholesterol,13.15±0.55μm/lens of total cholesterol and 0.13±0.06μm/lens of albumin. Using the cleaning and maintenance system Cleaning Drops® showed an efficacy for eliminating lipid deposits of 71% for blue light-filtering contact lens and 47% for non-blue lightfiltering contact lens. The efficacy for eliminating protein deposits was 60% for blue light-filtering contact lens and 93% for non blue light-filtering contact lens.
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