RT Journal Article
T1 Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation
A1 Illescas Martínez, Beatriz María
A1 Pérez Sánchez, Alfonso
A1 Mallo, Araceli
A1 Martín Doménech, Ángel
A1 Rodríguez Crespo, José Ignacio
A1 Martín León, Nazario
AB Non-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy. The synthesis of hexakis-adducts of [60]fullerene endowed with 12, 24 and 36 positive ammonium groups and a tridecafullerene appended with 120 positive charges has been performed. The delivery of a plasmid containing the green fluorescent protein (EGFP) gene into HEK293 (Human Embryonic Kidney) cells resulting in effective gene expression has demonstrated the efficacy of these compounds to form polyplexes with DNA. Particularly, giant tridecafullerene macromolecules have shown higher efficiency in the complexation and transfection of DNA. Thus, they can be considered as promising non-viral vectors for transfection purposes.
PB The Royal Society of Chemistry
SN 2050-750X
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/92879
UL https://hdl.handle.net/20.500.14352/92879
LA eng
NO Illescas, B. M.; Pérez-Sánchez, A.; Mallo, A.; Martín-Domenech, Á; Rodríguez-Crespo, I.; Martín, N. Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation. J. Mater. Chem. B 2020, 8, 4505-4515 DOI:10.1039/D0TB00113A.
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 1 sept 2024