RT Journal Article
T1 Identification of clinically relevant profiles in colorectal cancer through integrated analysis of bacterial DNA and metabolome in serum
A1 De la-Serna Esteban, Sofía Cristina
A1 Domínguez Serrano, María Inmaculada
A1 Dziakova, Jana
A1 Torres García, Antonio José
A1 Iniesta Serrano, María Pilar
A1 Vicente-Valor, Juan
A1 Tesolato, Sofía
A1 Gómez-Garre, Dulcenombre
A1 Paz-Cabezas, Mateo
A1 Ortega-Hernández, Adriana
A1 Fernández-Hernández, Constanza
A1 Rivera, Daniel
A1 Rupérez, Francisco-Javier
A1 García, Antonia
AB There is increasing evidence demonstrating the relationship between microbiota and colorectal cancer. Several studies have been published analyzing microbiota in tissues and feces from cancer patients; however, there are only a few publications investigating the clinical utility of serum microbiome from colorectal cancer patients. Our aim was to advance in the search for serum biomarkers for the diagnosis of colorectal cancer. We conducted a cross-sectional study assessing bacterial DNA and metabolomic profiles in 64 serum samples from subjects affected by colorectal cancer and controls. A metagenomic analysis of the bacterial 16S rRNA gene in serum was established, and serum metabolites were detected through an untargeted metabolic study based on Gas Chromatography-Quadruple Time-Of-Flight Mass Spectrometry with accurate mass. After integrating the data resulting from the bioinformatics and statistical analyses, we obtained different profiles in colorectal cancer population and controls, regardless of the subjects' age, gender and body mass index. Serum levels of Firmicutes and threonic acid were the most relevant characteristics that could help differentiate both groups, achieving an excellent predictive accuracy in this discovery cohort (area under the ROC curve = 0.95). Although these results should be validated in other cohorts through multicenter studies, we consider that our data could be relevant and applicable to the early diagnosis of colorectal cancer.
PB Frontiers Media
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/122682
UL https://hdl.handle.net/20.500.14352/122682
LA eng
NO Vicente-Valor J, Tesolato S, Gómez-Garre D, Paz-Cabezas M, Ortega-Hernández A, Fernández-Hernández C, de la Serna S, Domínguez-Serrano I, Dziakova J, Rivera D, Rupérez F-J, García A, Torres A and Iniesta P (2025) Identification of clinically relevant profiles in colorectal cancer through integrated analysis of bacterial DNA and metabolome in serum. Front. Immunol. 16:1562416. doi: 10.3389/fimmu.2025.1562416
NO The author(s) declare that financial support was received for the research and/or publication of this article. The present study was supported by grant PI19/00073 from the Carlos III Health Institute (Ministerio de Economıa y Competitividad), Spain, and by grant ́ PID2021-122490NB-I00, from the MICIN/AEI/10.13039/ 501100011033, both co-funded by the European Union through the European Regional Development Fund (ERDF) ‘A way to make Europe’.
NO Instituto de Salud Carlos III
NO Ministerio de Economía y Competitividad (España)
NO Agencia Estatal de Investigación (España)
NO European Commission
DS Docta Complutense
RD 30 dic 2025