%0 Book Section
%T Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)
publisher Springer
%D 2017
%U 978-1-4939-7182-4 (Print) 978-1-4939-7183-1 (Online)
%@ https://hdl.handle.net/20.500.14352/19465
%X Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.
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