RT Book, Section
T1 Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)
A1 Guevara, Govinda
A1 Perera González, Jesús
A1 Navarro Llorens, Juana María
AB Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.
PB Springer
SN 978-1-4939-7182-4 (Print) 978-1-4939-7183-1 (Online)
SN 1064-3745, ESSN: 1940-6029
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/19465
UL https://hdl.handle.net/20.500.14352/19465
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 25 ago 2025