RT Journal Article T1 Combining autohydrolysis and ionic liquid microwave treatment to enhance enzymatic hydrolysis of Eucalyptus globulus wood A1 Rigual Hernández, Victoria de los Ángeles A1 Santos, Tamara A1 Domínguez Toribio, Juan Carlos A1 Alonso Rubio, María Virginia A1 Oliet Pala, María Mercedes A1 Rodríguez Somolinos, Francisco AB The combination of autohydrolysis and ionic liquid microwave treatments of eucalyptus wood have been studied to facilitate sugar production in a subsequent enzymatic hydrolysis step. Three autohydrolysis conditions 7(150 °C, 175 °C and 200 °C) in combination with two ionic liquid temperatures (80 °C and 120 °C) were compared in terms of chemical composition, enzymatic digestibility and sugar production. Morphology was measured (using SEM) and the biomass surface was visualized with confocal fluorescence microscopy. The synergistic cooperation of both treatments was demonstrated, enhancing cellulose accessibility. At intermediate autohydrolysis conditions (175 °C) and low ionic liquid temperature (80 °C), a glucan digestibility of 84.4% was obtained. Using SEM micrographs, fractal dimension (as a measure of biomass complexity) and lacunarity (as a measure of homogeneity) were calculated before and after pretreatment. High fractals dimensions and low lacunarities correspond to morphologically complex and homogeneous samples, that are better digested by enzyme cocktails. PB Elsevier SN 0960-8524 YR 2018 FD 2018 LK https://hdl.handle.net/20.500.14352/92265 UL https://hdl.handle.net/20.500.14352/92265 LA eng NO Rigual, Victoria, et al. «Combining Autohydrolysis and Ionic Liquid Microwave Treatment to Enhance Enzymatic Hydrolysis of Eucalyptus Globulus Wood». Bioresource Technology, vol. 251, marzo de 2018, pp. 197-203. https://doi.org/10.1016/j.biortech.2017.12.034. NO Ministerio de Economía y Competitividad (España) DS Docta Complutense RD 6 abr 2025