RT Journal Article
T1 Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
A1 Calderón Bernal, Johan Manuel
A1 Fernández, Ana
A1 Arnal, José Luis
A1 Sanz Tejero, Celia
A1 Vela Alonso, Ana Isabel
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Cid Vázquez, María Dolores
AB Studies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS typing as well as by virulotyping. Three capsular types (A, B, F) and three LPS genotypes (L2, L3, L6) were identified. Capsular and LPS typing revealed a very low genetic diversity (GD = 0.02) among P. multocida, with most isolates belonging to genotype A:L3 (97.6%). Virulotyping identified seven virulence-associated gene profiles, with two profiles including 95.9% of the isolates. A subset of isolates was further characterized by MLST and PFGE. The sequence types ST79 and ST13 were the most frequently identified and were grouped into the same clonal complex (CC13), a result that supports the clonal population structure of BRD-associated P. multocida isolates. PFGE typing also revealed a low genetic diversity (GD = 0.18), detecting a single pattern in 62.5% of the outbreaks in which multiple isolates were analyzed. Overall, 85.2% of the isolates belonged to pulsotypes with at least 80% genetic similarity, consistent with a clonal population structure observed by MLST analysis and corroborating the genetic relatedness of most P. multocida isolates associated with BRD in cattle.
PB MPDI
SN 2076-2615
YR 2022
FD 2022-12-24
LK https://hdl.handle.net/20.500.14352/73198
UL https://hdl.handle.net/20.500.14352/73198
LA eng
NO Santander-Universidad Complutense de Madrid
DS Docta Complutense
RD 17 abr 2025