RT Journal Article
T1 Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque
A1 González-López, Paula
A1 Ares-Carral, Carla
A1 López-Pastor, Andrea R.
A1 Infante-Menéndez, Jorge
A1 González Illanes, Tamara
A1 Vega de Ceniga, Melina
A1 Esparza, Leticia
A1 Beneit, Nuria
A1 Martín-Ventura, José Luis
A1 Escribano Illanes, Óscar
A1 Gómez Hernández, María De La Almudena
AB (1) Background: Cardiovascular diseases (CVDs) are the main cause of death in developed countries, being atherosclerosis, a recurring process underlying their apparition. MicroRNAs (miRNAs) modulate the expression of their targets and have emerged as key players in CVDs; (2) Methods: 18 miRNAs were selected (Pubmed and GEO database) for their possible role in promoting atherosclerosis and were analysed by RT-qPCR in the aorta from apolipoprotein E-deficient (ApoE−/−) mice. Afterwards, the altered miRNAs in the aorta from 18 weeks-ApoE−/− mice were studied in human aortic and carotid samples; (3) Results: miR-155-5p was overexpressed and miR-143-3p was downregulated in mouse and human atherosclerotic lesions. In addition, a significant decrease in protein kinase B (AKT), target of miR-155-5p, and an increase in insulin-like growth factor type II receptor (IGF-IIR), target of miR-143-3p, were noted in aortic roots from ApoE−/− mice and in carotid plaques from patients with advanced carotid atherosclerosis (ACA). Finally, the overexpression of miR-155-5p reduced AKT levels and its phosphorylation in vascular smooth muscle cells, while miR-143-3p overexpression decreased IGF-IIR reducing apoptosis in vascular cells; (4) Conclusions: Our results suggest that miR-155-5p and miR-143-3p may be implicated in insulin resistance and plaque instability by the modulation of their targets AKT and IGF-IIR, contributing to the progression of atherosclerosis.
PB MDPI
SN 1661-6596
YR 2022
FD 2022-09-06
LK https://hdl.handle.net/20.500.14352/93718
UL https://hdl.handle.net/20.500.14352/93718
LA eng
NO González-López P, Ares-Carral C, López-Pastor AR, Infante-Menéndez J, González Illaness T, Vega De Ceniga M, et al. Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque. IJMS 2022;23:10253. https://doi.org/10.3390/ijms231810253.
NO Ministerio de Ciencia e Innovación y Universidades (España)
NO Banco Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 29 sept 2024