RT Journal Article
T1 Splitting of a prevalent Mycobacterium bovis spoligotype by variable-number tandem-repeat typing reveals high heterogeneity in an evolving clonal group
A1 Rodriguez Campos, Sabrina
A1 Navarro, Yurena
A1 Romero Martínez, Beatriz
A1 Juan Ferré, Lucía De
A1 Bezos Garrido, Javier
A1 Mateos García, Ana Isabel
A1 Golby, Paul
A1 Smith, Noel H
A1 Hewinson, Glyn R
A1 García de Viedma, Darío
A1 Aranaz Martín, Alicia
A1 Domínguez Rodríguez, Lucas José
AB Mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. This study applied mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing to a random panel of 115 M. bovis isolates that are representative of the most frequent spoligotype in the Iberian Peninsula, SB0121. VNTR typing targeted nine loci: ETR-A (alias VNTR2165), ETR-B (VNTR2461), ETR-D (MIRU4, VNTR580), ETR-E (MIRU31, VNTR3192), MIRU26 (VNTR2996), QUB11a (VNTR2163a), QUB11b (VNTR2163b), QUB26 (VNTR4052), and QUB3232 (VNTR3232). We found a high degree of diversity among the studied isolates (discriminatory index [D] = 0.9856), which were split into 65 different MIRU-VNTR types. An alternative short-format MIRU-VNTR typing targeting only the four loci with the highest variability values was found to offer an equivalent discriminatory index. Minimum spanning trees using the MIRU-VNTR data showed the hypothetical evolution of an apparent clonal group. MIRU-VNTR analysis was also applied to the isolates of 176 animals from 15 farms infected by M. bovis SB0121; in 10 farms, the analysis revealed the coexistence of two to five different MIRU types differing in one to six loci, which highlights the frequency of undetected heterogeneity.
SN 1098-660X
YR 2013
FD 2013-11
LK https://hdl.handle.net/20.500.14352/35287
UL https://hdl.handle.net/20.500.14352/35287
LA eng
NO Unión Europea. FP7
NO Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente
NO Fondo de Investigaciones Sanitarias (FIS)
DS Docta Complutense
RD 6 oct 2024