RT Journal Article
T1 Overcoming biochemical limitations of galactose oxidase through the design of a solid-supported self-sufficient biocatalyst
A1 Lorente-Arevalo, Alvaro
A1 Orellana Moraleda, Guillermo
A1 Laderi, Miguel
A1 Bolívar Bolívar, Juan Manuel
AB Galactose Oxidase (GalOx) has gained significant interest in biocatalysis due to its ability for selective oxidation beyond the natural oxidation of galactose, enabling the production of valuable derivatives. However, the practical application of GalOx has been hindered by the limited availability of active and stable biocatalysts, as well as the inherent biochemical limitations such as oxygen (O2) dependency and the need for activation. In this study, we addressed these challenges by immobilizing GalOx into agarose-based and Purolite supports to enhance its activity and stability. Additionally, we identified and quantified the oxygen supply limitation into solid catalysts by intraparticle oxygen sensing showing a trade-off between the amount of protein loaded onto the solid support and the catalytic effectiveness of the immobilized enzyme. Furthermore, we coimmobilized a heme-containing protein along with the enzyme to function as an activator. To evaluate the practical application of the immobilized GalOx, we conducted the oxidation of galactose in an instrumented aerated reactor. The results showcased the efficient performance of the immobilized enzyme in the 8 h reaction cycle. Notably, the GalOx immobilized into dextran sulfate-activated agarose exhibited improved stability, overcoming the need for a soluble activator supply, and demonstrated exceptional performance in galactose oxidation. These findings offer promising prospects for the utilization of GalOx in technical biocatalytic applications.
PB Wiley-VCH GmbH
SN 1439-4227
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/88993
UL https://hdl.handle.net/20.500.14352/88993
LA eng
NO Lorente-Arevalo A, Orellana G, Ladero M, Bolivar JM. Overcoming biochemical limitations of galactose oxidase through the design of a solid-supported self-sufficient biocatalyst. Chembiochem. 2023 Oct 2:e202300421. doi: 10.1002/cbic.202300421. Epub ahead of print. PMID: 37782555.
NO Comunidad de Madrid
NO European Commission
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 5 abr 2025