RT Journal Article
T1 Xerotolerance: a new property in Exiguobacterium Genus
T2 Xerotolerancia: Una nueva propiedad del género de Exiguobacterium
A1 Castillo López, María
A1 Galán, Beatriz
A1 Carmona, Manuel
A1 Navarro Llorens, Juana María
A1 Peretó, Juli
A1 Porcar, Manuel
A1 Getino, Luis
A1 Olivera, Elías R.
A1 Luengo, José M.
A1 García, José Luis
A1 Castro Ruiz, Laura
AB The highly xerotolerant bacterium classified as Exiguobacterium sp. Helios isolated from a solar panel in Spain showed a close relationship to Exiguobacterium sibiricum 255-15 isolated from Siberian permafrost. Xerotolerance has not been previously described as a characteristic of the extremely diverse Exiguobacterium genus, but both strains Helios and 255-15 showed higher xerotolerance than that described in the reference xerotolerant model strain Deinococcus radiodurans. Significant changes observed in the cell morphology after their desiccation suggests that the structure of cellular surface plays an important role in xerotolerance. Apart from its remarkable resistance to desiccation, Exiguobacterium sp. Helios strain shows several polyextremophilic characteristics that make it a promising chassis for biotechnological applications. Exiguobacterium sp. Helios cells produce nanoparticles of selenium in the presence of selenite linked to its resistance mechanism. Using the Lactobacillus plasmid pRCR12 that harbors a cherry marker, we have developed a transformation protocol for Exiguobacterium sp. Helios strain, being the first time that a bacterium of Exiguobacterium genus has been genetically modified. The comparison of Exiguobacterium sp. Helios and E. sibiricum 255-15 genomes revealed several interesting similarities and differences. Both strains contain a complete set of competence-related DNA transformation genes, suggesting that they might have natural competence, and an incomplete set of genes involved in sporulation; moreover, these strains not produce spores, suggesting that these genes might be involved in xerotolerance.
PB MDPI
SN Electronic: 2076-2607
YR 2021
FD 2021-11-28
LK https://hdl.handle.net/20.500.14352/4902
UL https://hdl.handle.net/20.500.14352/4902
LA eng
NO López MC, Galán B, Carmona M, Navarro Llorens JM, Peretó J, Porcar M, Getino L, Olivera ER, Luengo JM, Castro L, García JL. Xerotolerance: A New Property in Exiguobacterium Genus. Microorganisms. 2021 Nov 28;9(12):2455. doi: 10.3390/microorganisms9122455. PMID: 34946057; PMCID: PMC8706201.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Consejo Superior de Investigaciones Científicas (España)
NO Agencia Estatal de Investigación (España)
DS Docta Complutense
RD 6 abr 2025