RT Journal Article
T1 Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro
A1 Chamorro, Eva
A1 Muñoz de Luna, Javier
A1 Bonnin Arias, Cristina Natalia
A1 Pérez Carrasco, María Jesús
A1 Vázquez Molini, Daniel
A1 Sánchez Ramos, Celia
AB Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.
PB Wiley
SN 0031-8655
SN 1751-1097
YR 2012
FD 2012-10-09
LK https://hdl.handle.net/20.500.14352/99624
UL https://hdl.handle.net/20.500.14352/99624
LA eng
NO Chamorro E, Bonnin-Arias C, Pérez-Carrasco MJ, Muñoz de Luna J, Vázquez D, Sánchez-Ramos C. Effects of light-emitting diode radiations on human retinal pigment epithelial cells in vitro. Photochem Photobiol. 2013 Mar-Apr;89(2):468-73. doi: 10.1111/j.1751-1097.2012.01237.x. Epub 2012 Oct 9. PMID: 22989198.
NO Issue Online: 01 March 2013; Version of Record online: 09 October 2012; Accepted manuscript online: 18 September 2012; Manuscript accepted: 30 August 2012; Manuscript received: 26 June 2012
NO Fundación Mapfre (España)
DS Docta Complutense
RD 6 abr 2025