RT Journal Article
T1 Selective oxidation of glycerol to 1,3-dihydroxyacetone by covalently immobilized glycerol dehydrogenases with higher stability and lower product inhibition
A1 Rocha Martín, Javier
A1 Acosta, Andreína
A1 Berenguer, José
A1 Guisán, José
A1 López-Gallego, Fernando
AB Glycerol dehydrogenase (GlyDH) catalyzes the regioselective oxidation of glycerol to yield 1,3-dihydroxyacetone (DHA); an important building block in chemical industry. Three recombinant GlyDHs from Geobacillus stearothermophilus, from Citrobacter braakii and from Cellulomonas sp. were stabilized by covalent immobilization. The highest activity recoveries (40–50%) of the insoluble preparations were obtained by immobilizing these enzymes in presence of polyethylene glycol (PEG). Noteworthy, these immobilized preparations were more stable and less inhibited by DHA than their soluble counterparts. In particular, GlyDH from G. stearothermophilus immobilized on agarose activated with both amine and glyoxyl groups and crosslinked with dextran aldehyde was 3.7-fold less inhibited by DHA than its soluble form and retained 100% of its initial activity after 18 h of incubation at 65 °C and pH 7. This is one of the few examples where the same immobilization protocol has minimized enzyme product inhibition and maximized thermal stability.
PB Elsevier
SN 0960-8524
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/94101
UL https://hdl.handle.net/20.500.14352/94101
LA eng
NO Rocha-Martin, Javier, et al. «Selective Oxidation of Glycerol to 1,3-Dihydroxyacetone by Covalently Immobilized Glycerol Dehydrogenases with Higher Stability and Lower Product Inhibition». Bioresource Technology, vol. 170, octubre de 2014, pp. 445-53. https://doi.org/10.1016/j.biortech.2014.07.116.
NO FundingEste trabajo ha contado con el apoyo del Ministerio de Economía e Innovación de España (proyecto CTQ2009-07568 ). Queremos agradecer a IKERBASQUE , Fundación Vasca para la Ciencia por la financiación al doctor Fernando López Gallego. También agradecemos al gobierno de la Comunidad de Madrid por financiar la beca de doctorado de J. Rocha-Martín.
NO Ministerio de Economía e Innovación (España)
NO Comunidad de Madrid
NO Fundación Vasca para la Ciencia 
DS Docta Complutense
RD 9 abr 2025