RT Journal Article
T1 Tumor-infiltrating b-and T-cell repertoire in pancreatic cancer associated with host and tumor features
A1 Pineda Sanjuan, Silvia
A1 López de Maturana, Evangelina
A1 Yu, Katharine
A1 Ravoor, Akshay
A1 Wood, Inés
A1 Malats, Núria
A1 Sirota, Marina
A2 Notarangelo, Luigi Daniele
AB Background: Infiltrating B and T cells have been observed in several tumor tissues, including pancreatic ductal adenocarcinoma (PDAC). The majority known PDAC risk factors point to a chronic inflammatory process leading to different forms of immunological infiltration. Understanding pancreatic tumor infiltration may lead to improved knowledge of this devastating disease.Methods: We extracted the immunoglobulins (IGs) and T cell receptors (TCRs) from RNA-sequencing of 144 PDAC from TCGA and 180 pancreatic normal tissue from GTEx. We used Shannon entropy to find differences in IG/TCR diversity. We performed a clonotype analysis considering the IG clone definition (same V and J segments, same CDR3 length, and 90% nucleotide identity between CDR3s) to study differences among the tumor samples. Finally, we performed an association analysis to find host and tumor factors associated with the IG/TCR.Results: PDAC presented a richer and more diverse IG and TCR infiltration than normal pancreatic tissue. A higher IG infiltration was present in heavy smokers and females and it was associated with better overall survival. In addition, specific IG clonotypes classified samples with better prognosis explaining 24% of the prognosis phenotypic variance. On the other hand, a larger TCR infiltration was present in patients with previous history of diabetes and was associated with lower nonantigen load.Conclusions: Our findings support PDAC subtyping according to its immune repertoire landscape with a potential impact on the understanding of the inflammatory basis of PDAC risk factors as well as the design of treatment options and prognosis monitoring
PB Frontiers Research Foundation
SN 1664-3224
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/94490
UL https://hdl.handle.net/20.500.14352/94490
LA eng
NO Pineda S, López de Maturana E, Yu K, Ravoor A, Wood I, Malats N and Sirota M (2021) Tumor-Infiltrating B- and T-Cell Repertoire in Pancreatic Cancer Associated With Host and Tumor Features. Front. Immunol. 12:730746.
NO American Association for Cancer Research
NO Ministerio de Sanidad (España)
NO Instituto de Salud Carlos III
NO Lustgarten Foundation & Stand-Up to Cancer
DS Docta Complutense
RD 10 abr 2025