RT Book, Section
T1 Low-density primary cell culture of postnatal murine cerebellar progenitors in vitro
A1 Llorente-Sáez, Celia
A1 Serrano-López, Julia
A1 García Delicado, Esmerilda
A1 Pérez Sen, Raquel
A1 Gómez Villafuertes, María Rosa
A1 Ortega De La O, Felipe
A2 Ortega De La O, Felipe
AB Cerebellum is the major player of motor functions of the body, as well as being involved in plenty of nonmotor behavior traits. There are numerous disorders related to cerebellum that have severe consequences for patients and the absence of an effective treatment, so it is crucial to emphasize conducting research directed to deeply understand the biology of this structure, giving special importance to stem cells that could have regenerative potential. Here, we describe a novel protocol for isolating neural stem cells from postnatal mouse cerebellum, allowing for the study of progenitor cells from three distinct proliferative niches. Cells are maintained in low-density cultures without external growth factors, facilitating the study of intrinsic programming. We also suggest numerous applications that could provide an insight into the identity, development, and behavior of progenitor cells, which may contribute to the development of treatments for cerebellar disorders.
PB Springer US
SN 9781071643853
SN 9781071643860
SN 1064-3745
SN 1940-6029
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/128627
UL https://hdl.handle.net/20.500.14352/128627
LA eng
NO Llorente-Sáez, C., Serrano-López, J., Delicado, E. G., Pérez-Sen, R., Gómez-Villafuertes, R., & Ortega, F. (2025). Low-Density Primary Cell Culture of Postnatal Murine Cerebellar Progenitors In Vitro. Methods in molecular biology (Clifton, N.J.), 2899, 35–46. https://doi.org/10.1007/978-1-0716-4386-0_3
NO Ministerio de Ciencia e Innovación y Universidades (España)
NO Banco Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 26 feb 2026