RT Journal Article
T1 Controlled pDNA release in gemini cationic lipoplexes by femtosecond laser irradiation of gold nanostars
A1 Sánchez Arribas, Natalia
A1 Díaz-Núñez, Pablo
A1 Osío Barcina, José De Jesús
A1 Aicart Sospedra, Emilio
A1 Junquera González, María Elena
A1 Guerrero Martínez, Andrés
AB The design of nanovectors able to overcome biological barriers is one of the main challenges in biomedicine. Gemini cationic lipids are considered potential candidates for gene therapy due to their high biocompatibility and capacity to condense nucleic acids safely in the form of lipoplexes. However, this approach presents difficulties regarding genetic unpacking and, therefore, control over this process becomes crucial to ensure successful transfection. In this work, gemini cationic lipoplexes were prepared in the presence of plasmonic gold nanostars (AuNSs) to afford a nanovector that efficiently releases plasmid DNA (pDNA) upon irradiation with near-infrared femtosecond laser pulses. A critical AuNSs concentration of 50 pM and optimized laser power density of 400 mW led to successful pDNA release, whose efficiency could be further improved by increasing the irradiation time. Agarose gel electrophoresis was used to confirm pDNA release. UV-Vis-NIR spectroscopy and transmission electron microscopy studies were performed to monitor changes in the morphology of the AuNSs and lipoplexes after irradiation. From a physicochemical point of view, this study demonstrates that the use of AuNSs combined with gemini cationic lipoplexes allows control over pDNA release under ultrafast laser irradiation.
PB MDPI
SN 2079-4991
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/108923
UL https://hdl.handle.net/20.500.14352/108923
LA eng
NO Sánchez-Arribas, N.; Díaz-Núñez, P.; Osío Barcina, J.; Aicart, E.; Junquera, E.; Guerrero-Martínez, A. Controlled pDNA Release in Gemini Cationic Lipoplexes by Femtosecond Laser Irradiation of Gold Nanostars. Nanomaterials 2021, 11, 1498. https:// doi.org/10.3390/nano11061498
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Universidad Complutense de Madrid
NO Comunidad Autónoma de Madrid
DS Docta Complutense
RD 10 abr 2025