RT Journal Article
T1 Distribution of the transcription factor islet-1 in the central nervous system of nonteleost actinopterygian fish:Relationshipwith cholinergic and catecholaminergic systems
A1 Lozano Rebollo, Daniel
A1 Moreno García, Nerea
A1 Jiménez Álvarez, Sara
A1 Chinarro Sánchez, Adrián
A1 Morona Arribas, Ruth
A1 López Redondo, Jesús María
AB Islet-1 (Isl1) is one of the most conserved transcription factors in the evolution of vertebrates, due to its continuing involvement in such important functions as the differentiation of motoneurons, among other essential roles in cell fate in the forebrain. Although its functions are thought to be similar in all vertebrates, the knowledgeabout the conservation of its expression pattern in the central nervous system goes as far as teleosts, leaving the basal groups of actinopterygian fishes overlooked, despitetheir important phylogenetic position. In order to assess the extent of its conservation among vertebrates, we studied its expression pattern in the central nervous system ofselected nonteleost actinopterygian fishes. By means of immunohistochemical techniques, we analyzed the Isl1 expression in the brain, spinal cord, and sensory gangliaof the cranial nerves of young adult specimens of the cladistian species Polypterus senegalus and Erpetoichthys calabaricus, the chondrostean Acipenser ruthenus, and theholostean Lepisosteus oculatus. We also detected the presence of the transcription factor Orthopedia and the enzymes tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) to better locate all the immunoreactive structures in the different brainareas and to reveal the possible coexpression with Isl1. Numerous conserved features in the expression pattern of Isl1 were observed in these groups of fishes, suchas populations of cells in the subpallial nuclei, preoptic area, subparaventricular and tuberal hypothalamic regions, prethalamus, epiphysis, cranial motor nuclei and sensoryganglia of the cranial nerves, and the ventral horn of the spinal cord. Double labeling of TH and Isl1 was observed in cells of the preoptic area, the subparaventricular andtuberal hypothalamic regions, and the prethalamus, while virtually all motoneurons in the hindbrain and the spinal cord coexpressed ChAT and Isl1. Altogether, these resultsshow the high degree of conservation of the expression pattern of the transcription factor Isl1, not only among fish, but in the subsequent evolution of vertebrates.
PB Wiley
SN 0021-9967
YR 2023
FD 2023-04-18
LK https://hdl.handle.net/20.500.14352/72413
UL https://hdl.handle.net/20.500.14352/72413
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Universidad Complutense de Madrid (UCM)
DS Docta Complutense
RD 5 abr 2025