RT Journal Article
T1 Response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces
A1 Sánchez Beltrán, María Del Carmen
A1 Fernández, Eva
A1 Llama Palacios, María Arantxazu
A1 Figuero Ruiz, Elena
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB ObjectiveThe aim of this study was to develop in vitro biofilms on SLA titanium (Ti-SLA) and zirconium oxide (ZrO2) surfaces and to evaluate the effect of antiseptic agents on the number of putative periodontal pathogenic species.MethodsAn in vitro biofilm model was developed on sterile discs of Ti-SLA and ZrO2. Three antiseptic agents [chlorhexidine and cetyl-pyridinium-chloride (CHX/CPC), essential oils (EEOOs) and cetyl-peridinium-chloride (CPC)] were applied to 72-h biofilms, immersing discs during 1 min in the antiseptic solution, either with or without mechanical disruption. Viable bacteria [colony forming units (CFU/mL)] were measured by quantitative polymerase chain reaction (qPCR) combined with propidium monoazide. A generalized lineal model was constructed to determine the effect of the agents on the viable bacterial counts of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Fusobacterium nucleatum on each surface.ResultsThe exposure to each antiseptic solution resulted in a statistically significant reductions in the number of viable target species included in the in vitro multi-species biofilm, on both Ti-SLA and ZrO2 (p < 0.001) which was of up to 2 orders for A. actinomycetemcomitans, for P. gingivalis 2 orders on Ti-SLA and up to 3 orders on ZrO2, and, for F. nucleatum up to 4 orders. No significant differences were found in counts of the tested bacteria between in vitro biofilms formed on both Ti-SLA and ZrO2, after topically exposure to the antimicrobial agents whether the application was purely chemical or combined with mechanical disruption.SignificanceA. actinomycetemcomitans, P. gingivalis and F. nucleatum responded similarly to their exposure to antiseptics when grown in multispecies biofilms on titanium and zirconium surfaces, in spite of the described structural differences between these bacterial communities.
PB Elsevier
SN 0109-5641
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/95183
UL https://hdl.handle.net/20.500.14352/95183
LA eng
NO M.C. Sánchez, E. Fernández, A. Llama-Palacios, E. Figuero, D. Herrera, M. Sanz, Response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces, Dental Materials, Volume 33, Issue 4, 2017, p. 446-453, https://doi.org/10.1016/j.dental.2017.01.013.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO DENTAID
DS Docta Complutense
RD 6 abr 2025