TY - JOUR AU - Bolívar Bolívar, Juan Manuel AU - Rocha-Martin, Javier AU - Mateo, Cesar AU - Cava, Felipe AU - Berenguer, Jose AU - Vega, Daniel AU - Fernandez-Lafuente, Roberto AU - Guisan, Jose M. PY - 2009 DO - 10.1016/j.molcatb.2008.12.010 SN - 1381-1177 UR - https://hdl.handle.net/20.500.14352/93132 T2 - Journal of Molecular Catalysis B: Enzymatic AB - The immobilization of a glutamate dehydrogenase from Thermus thermophilus (GDH) on glyoxyl agarose beads at pH 7 has permitted to perform the immobilization, purification and stabilization of this interesting enzyme. It was cloned in Escherichia coli... LA - eng M2 - 158 PB - Elsevier KW - Thermophilic enzymes KW - Multimeric enzymes KW - Enzyme stabilization KW - Enzyme immobilization KW - Protein purification KW - Oriented immobilization KW - Multipoint covalent attachment TI - Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization TY - journal article VL - 58 ER -