RT Journal Article
T1 Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging
A1 Santos, Harrison D. A.
A1 Zabala Gutiérrez, Irene
A1 Shen, Yingli
A1 Lifante, José
A1 Ximendes, Erving
A1 Laurenti, Marco
A1 Méndez González, Diego
A1 Melle Hernández, Sonia
A1 Gómez Calderón, Óscar
A1 López Cabarcos, Enrique
A1 Fernández Monsalve, Nuria
A1 Chavez Coria, Irene
A1 Lucena Agell, Daniel
A1 Monge, Luis
A1 Mackenzie, Mark D.
A1 Marqués Hueso, José
A1 Jones, Callum M. S.
A1 Jacinto, Carlos
A1 Rosal, Blanca, del
A1 Kar, Ajoy K.
A1 Rubio Retama, Jorge
A1 Jaque García, Daniel
AB Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm−2) and doses (<0.5 mg kg−1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.
PB Nature Publishing Group
SN 2041-1723
YR 2020
FD 2020-06-10
LK https://hdl.handle.net/20.500.14352/6329
UL https://hdl.handle.net/20.500.14352/6329
LA eng
NO Received: 11 May 2019; Accepted: 24 April 2020; Published: 10 June 2020
NO Unión Europea. Horizonte 2020
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid/FEDER
NO Universidad Complutense de Madrid/Banco de Santander
NO China Scholarship Council
NO Fundación para la Investigación Biomédica del Hospital Universitario Ramón y Cajal (España)
NO UK Engineering and Physical Sciences Research Council
NO Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
NO Financiadora de Estudos e Projetos (Brasil)
NO Fundação de Amparo à Pesquisa do Estado de Alagoas (Brasil)
NO Universidad Autónoma de Madrid (España)
DS Docta Complutense
RD 17 ago 2024