RT Journal Article T1 In situ use of mining substrates for wetland construction: results of a pilot experiment A1 Hernández Pérez, Carmen A1 Martínez López, Salvadora A1 Martínez Sánchez, Maria José A1 Martínez Martínez, Lucía Belén A1 García Lorenzo, María de la Luz A1 Pérez Sirvent, Carmen AB This paper evaluates an experimental wetland as part of a pilot soil reclamation project in a mining area. The wetland was constructed using materials of mining origin from the area; most reactive materials of acid pH were stabilised using limestone filler. The study selected macrophytes that are tolerant to potentially toxic elements (PTEs) and resistant to salinity, namely Phragmites australis, Juncus effusus, and Iris pseudacorus. These macrophytes were then placed in pots containing substrates composed of different mixtures of topsoil, peat, and mining waste (black or yellow sand). A thorough analysis of the physicochemical and mineralogical characteristics of the materials included studies of PTE mobilisation. This study emphasises the significance of the rhizosphere in directing the transfer of PTEs to the plant and the correlation between the substrate and the development of plant defence mechanisms, such as the formation of Fe-plates. Scanning electron microscopy was used to highlight these aspects and validate the results of the analytical determinations. These wetlands can be proposed as a phytoremediation strategy for areas affected by mining and maritime influence. They are easy to construct and remain stable, providing important ecosystem services such as the natural attenuation of acid mine drainage, support for vegetation development and fauna, and a clean ecosystem. PB MDPI YR 2024 FD 2024-04-22 LK https://hdl.handle.net/20.500.14352/104198 UL https://hdl.handle.net/20.500.14352/104198 LA eng NO Hernández-Pérez, Carmen, et al. «In Situ Use of Mining Substrates for Wetland Construction: Results of a Pilot Experiment». Plants, vol. 13, n.o 8, abril de 2024, p. 1161 DS Docta Complutense RD 22 abr 2025