RT Journal Article
T1 <i>In vivo</i> and <i>in vitro</i> maturation of rabbit oocytes differently affects the gene expression profile, mitochondrial distribution, apoptosis and early embryo development
A1 López-Tello, J.
A1 Arias Álvarez, María
A1 García García, Rosa María
A1 Rebollar, P.G.
A1 Gutiérrez Adán, A.
A1 Lorenzo González, Pedro Luis
AB In vivo-matured cumulus–oocyte complexes are valuable models in which to assess potential biomarkers of rabbit oocyte quality that contribute to enhanced IVM systems. In the present study we compared some gene markers of oocytes and cumulus cells (CCs) from immature, in vivo-matured and IVM oocytes. Moreover, apoptosis in CCs, nuclear maturation, mitochondrial reallocation and the developmental potential of oocytes after IVF were assessed. In relation tocumulus expansion, gene expression of gap junction protein, alpha 1, 43 kDa (Gja1) and prostaglandin-endoperoxide synthase 2 (Ptgs2) wassignificantly lower in CCs after in vivo maturation than IVM. In addition, there were differences ingene expression after in vivo maturation versus IVM in both oocytes and CCs for genes related to cell cycle regulation and apoptosis (V-Akt murine thymoma viral oncogene homologue 1 (Akt1), tumour protein 53 (Tp53), caspase 3, apoptosisrelated cysteine protease (Casp3)), oxidative response (superoxide dismutase 2, mitochondrial (Sod2)) and metabolism (glucose-6-phosphate dehydrogenase (G6pd), glyceraldehyde-3-phosphate dehydrogenase (Gapdh)). In vivo-matured CCs had a lower apoptosis rate than IVM and immature CCs. Meiotic progression, mitochondrial migration to the periphery and developmental competence were higher for in vivo-matured than IVM oocytes. In conclusion, differences in oocyte developmental capacity after IVM or in vivo maturation are accompanied by significant changes in transcript abundance in oocytes and theirsurrounding CCs, meiotic rate, mitochondrial distribution and apoptotic index. Some of the genes investigated, such as Gja1, could be potential biomarkersfor oocyte developmental competence in the rabbit model, helping improve in vitro culture systems in these species.
PB Csiro
SN 1448-5990
YR 2016
FD 2016-09-26
LK https://hdl.handle.net/20.500.14352/102766
UL https://hdl.handle.net/20.500.14352/102766
LA eng
DS Docta Complutense
RD 10 abr 2025