RT Journal Article T1 Enhanced resistance to Botrytis cinerea in genetically-modified Vitis vinifera L. plants over-expressing the grapevine stilbene synthase gene A1 Dabauza, Mercedes A1 Velasco, Leonardo A1 Pazos-Navarro, María A1 Pérez-Benito, Ernesto A1 Hellín, Pilar A1 Flores, Pilar A1 Gómez Garay, Aranzazu A1 Martínez, Mari Carmen A1 Lacasa, Alfredo AB Grapevine (Vitis vinifera L.) was genetically modified with a construct containing a cDNA insert encoding the stilbene synthase gene (Vst1) from grapevine, under the control of the cauliflower mosaic virus 35S promoter in order to test the potential of over-production of resveratrol to protect plants from fungal attack. Southern blot hybridization and quantitative real-time PCR analysis demonstrated the presence and integration of one copy of exogenous DNA sequences in two grapevine-modified lines. Relative expression of the Vst1 gene in different modified lines was confirmed by using gene-specific quantitative real-time PCR. Compared to the control, the concentration of trans-resveratrol quantified by HPLC was up to 7.5 fold higher in the modified plants. The necrotic lesion size of leaves of intact modified plants inoculated by Botrytis cinerea B05.10 strain was consistently smaller and significantly different (p B 0.05) than in control plants, showing that modified grapevine plants were more resistant to the pathogen than the control plants. PB Springer Verlag SN 0167-6857, ESSN: 1573-5044 YR 2015 FD 2015-01 LK https://hdl.handle.net/20.500.14352/35435 UL https://hdl.handle.net/20.500.14352/35435 LA eng NO Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (España) NO Fondo Social Europeo DS Docta Complutense RD 12 abr 2025