RT Journal Article T1 Changes in polyphenol and polysaccharide content of grape seed extract and grape pomace after enzymatic treatment A1 Chamorro Francisco, Susana A1 Viveros Montoro, Agustín A1 Álvarez, Inmaculada A1 Vega, Estela A1 Brenes, Agustín AB Grape seed extract and grape pomace are rich sources of polyphenols. The aim of this study was to evaluate the release of polyphenols, the solubilisation of carbohydrate, and the antioxidant capacity of these grape by-products after enzymatic reaction with carbohydrases (cellulolytic and pectinolytic activities) and tannase for 24 h. The use of tannase in these by-products, and pectinase in grape pomace changed the galloylated form of catechin to its free form, releasing gallic acid and increasing the antioxidant activity. In grape pomace, cellulase treatment was not efficient for phenolic release and antioxidant activity improvement. The addition of carbohydrases to grape pomace, either alone or in combination, degraded the cell wall polysaccharides, increasing the content of monosaccharides. These results provide relevant data about the potential of pectinase, tannase and combinations of enzymes on the release of polyphenols and monosaccharides from grape by-products, improving the antioxidant capacity and the nutritional value. PB Elsevier SN 0308-8146 YR 2012 FD 2012 LK https://hdl.handle.net/20.500.14352/97573 UL https://hdl.handle.net/20.500.14352/97573 LA eng NO Chamorro, S., et al. «Changes in Polyphenol and Polysaccharide Content of Grape Seed Extract and Grape Pomace after Enzymatic Treatment». Food Chemistry, vol. 133, n.o 2, julio de 2012, pp. 308-14. https://doi.org/10.1016/j.foodchem.2012.01.031. NO Ministerio de Ciencia e Innovación (España) NO Comunidad de Madrid DS Docta Complutense RD 22 abr 2025