RT Journal Article
T1 Arabidopsis cell wall composition determines disease resistance specificity and fitness
A1 Molina, Antonio
A1 Miedes, Eva
A1 Bacete, Laura
A1 Rodríguez González, Juan Tinguaro
A1 Mélida, Hugo
A1 Denancé, Nicolas
A1 Sánchez-Vallet, Andrea
A1 Rivière, Marie-Pierre
A1 López, Gemma
A1 Freydier, Amandine
A1 Barlet, Xavier
A1 Pattathil, Sivakumar
A1 Hahn, Michael
A1 Goffner, Deborah
AB Plant cell walls are complex structures subject to dynamic remodeling in response to developmental and environmental cues and play essential functions in disease resistance responses. We tested the specific contribution of plant cell walls to immunity by determining the susceptibility of a set of Arabidopsis cell wall mutants (cwm) to pathogens with different parasitic styles: a vascular bacterium, a necrotrophic fungus, and a biotrophic oomycete. Remarkably, most cwm mutants tested (29/34; 85.3%) showed alterations in their resistance responses to at least one of these pathogens in comparison to wild-type plants, illustrating the relevance of wall composition in determining disease-resistance phenotypes. We found that the enhanced resistance of cwm plants to the necrotrophic and vascular pathogens negatively impacted cwm fitness traits, such as biomass and seed yield. Enhanced resistance of cwm plants is not only mediated by canonical immune pathways, like those modulated by phytohormones or microbe-associated molecular patterns, which are not deregulated in the cwm tested. Pectin-enriched wall fractions isolated from cwm plants triggered immune responses in wild-type plants, suggesting that wall-mediated defensive pathways might contribute to cwm resistance. Cell walls of cwm plants show a high diversity of composition alterations as revealed by glycome profiling that detect specific wall carbohydrate moieties. Mathematical analysis of glycome profiling data identified correlations between the amounts of specific wall carbohydrate moieties and disease resistance phenotypes of cwm plants. These data support the relevant and specific function of plant wall composition in plant immune response modulation and in balancing disease resistance/development trade-offs.
PB National Academy of Sciences
SN 0027-8424
SN 1091-6490
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/114554
UL https://hdl.handle.net/20.500.14352/114554
LA eng
NO SignificancePlant cells are surrounded by an extracellular matrix known as the cell wall. We have analyzed the contribution of the Arabidopsis cell wall to disease resistance to pathogens with different parasitic styles. Here, we demonstrate that plant cell walls are determinants of immune responses since modification of their composition in a set of Arabidopsis cell wall mutants has an impact on their disease resistance and fitness phenotypes. In these genotypes, we identified specific correlations between the amounts of specific wall carbohydrate epitopes and disease resistance/fitness phenotypes through mathematical analyses. These data support the relevant and specific function of plant cell wall composition in plant immune responses and provide the basis for using wall traits in crop breeding programs.
NO Ministerio de Economía y Competitividad
NO Ministerio de Ciencia, Innovación y Universidades
NO Agence Nationale de la Recherche
DS Docta Complutense
RD 6 abr 2025