RT Journal Article
T1 Identification of Major Allergens in Watermelon
A1 Pastor Vargas, Carlos
A1 Cuesta-Herranz, Javier
A1 Cases, Barbara
A1 Pérez-Gordo, Marina
A1 Figueredo, Elena
A1 Heras González, Manuel De Las
A1 Vivanco Martínez, Fernando
AB Background: Watermelon is a worldwide consumed Cucurbitaceae fruit that can elicit allergic reactions. However, the major allergens of watermelon are not known. The aim of this study is to identify and characterize major allergens in watermelon.Methods: Twenty-three patients allergic to watermelon took part in the study. The diagnosis was based on a history of symptoms and positive skin prick-prick tests to watermelon, confirmed by positive open oral challenge testing to watermelon pulp. Allergenic components were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by N-terminal amino acid sequencing and mass spectrometry. Allergens were purified combining several chromatographic steps.Results: Several IgE binding bands (8-120 kDa) were detected in watermelon extract. Three major allergens were identified as malate dehydrogenase (36 kDa), triose phosphate isomerase (28 kDa) and profilin (13 kDa). Purified allergens individually inhibited IgE binding to the whole watermelon extract.Conclusions: All in all these results indicate that malate dehydrogenase, triose phosphate isomerase and profilin are major allergens involved in watermelon allergy.
PB Karger
SN 1018-2438
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/93097
UL https://hdl.handle.net/20.500.14352/93097
LA eng
NO Pastor C, Cuesta-Herranz J, Cases B, Pérez-Gordo M, Figueredo E, de las Heras M, Vivanco F. Identification of major allergens in watermelon. Int Arch Allergy Immunol. 2009;149(4):291-8. doi: 10.1159/000205574. Epub 2009 Mar 17. PMID: 19295232.
NO Ministerio de Educación y Ciencia (España)
NO Vegetalia Network
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 31 ago 2024