RT Journal Article
T1 Gene expression and regulatory factors of the mechanistictarget of rapamycin (mTOR) complex 1 predict mammalian longevity
A1 Mota Martorell, Natalia
A1 Jové, Mariona
A1 Pradas, Irene
A1 Berdún, Rebeca
A1 Sánchez, Isabel
A1 Naudí, Alba
A1 Gari, Eloi
A1 Barja de Quiroga, Gustavo
A1 Pamplona, Reinald
AB Species longevity varies significantly across animal species, but the underlying molecular mechanisms remain poorly understood. Recent studies and omics approaches suggest that phenotypic traits of longevity could converge in the mammalian target of rapamycin (mTOR) signalling pathway. The present study focuses on the comparative approach in heart tissue from 8 mammalian species with a ML ranging from 3.5 to 46 years. Gene expression, protein content, and concentration of regulatory metabolites of the mTOR complex 1 (mTORC1) were measured using droplet digital PCR, western blot, and mass spectrometry, respectively. Our results demonstrate (1) the existence of differences in species-specific gene expression and protein content of mTORC1, (2) that the achievement of a high longevity phenotype correlates with decreased and inhibited mTORC1, (3) a decreased content of mTORC1 activators in long-lived animals, and (4) that these differences are independent of phylogeny. Our findings, taken together, support an important role for mTORC1 downregulation in the evolution of longlived mammals.
PB Springer
SN 2509-2715, ESSN 2509-2723
YR 2020
FD 2020-06
LK https://hdl.handle.net/20.500.14352/6654
UL https://hdl.handle.net/20.500.14352/6654
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Ministerio de Ciencia, Innovación y Universidades (MCIU)
NO Generalitat de Catalunya. Agencia de Gestión de Ayudas Universitarias y de Investigación
NO Generaltitat de Catalunya. Departamento de Salud
NO Fondo Europeo de Desarrollo Regional (FEDER)
DS Docta Complutense
RD 1 may 2024