RT Journal Article
T1 Achieving low-power single-wavelength-pair nanoscopy with NIR-II continuous-wave laser for multi-chromatic probes
A1 Guo, Xin
A1 Pu, Rui
A1 Zhu, Zhimin
A1 Qiao, Shuqian
A1 Liang, Yusen
A1 Huang, Bingru
A1 Liu, Haichun
A1 Labrador Páez, Lucía
A1 Kostiv, Uliana
A1 Zhao, Pu
A1 Wu, Qiusheng
A1 Widengren, Jerker
A1 Zhan, Qiuqiang
AB Stimulated emission depletion (STED) microscopy is a powerful diffraction-unlimited technique for fluorescence imaging. Despite its rapid evolution, STED fundamentally suffers from high-intensity light illumination, sophisticated probe defined laser schemes, and limited photon budget of the probes. Here, we demonstrate a versatile strategy, stimulated-emission induced excitation depletion (STExD), to deplete the emission of multi-chromatic probes using a single pair of low-power, near-infrared (NIR), continuous-wave (CW) lasers with fixed wavelengths. With the effect of cascade amplified depletion in lanthanide upconversion systems, we achieve emission inhibition for a wide range of emitters (e.g., Nd3+, Yb3+, Er3+, Ho3+, Pr3+, Eu3+, Tm3+, Gd3+, and Tb3+) by manipulating their common sensitizer, i.e., Nd3+ ions, using a 1064-nm laser. With NaYF4:Nd nanoparticles, we demonstrate an ultrahigh depletion efficiency of 99.3 ± 0.3% for the 450 nm emission with a low saturation intensity of 23.8 ± 0.4 kW cm−2. We further demonstrate nanoscopic imaging with a series of multi-chromatic nanoprobes with a lateral resolution down to 34 nm, two-color STExD imaging, and subcellular imaging of the immunolabelled actin filaments. The strategy expounded here promotes single wavelength-pair nanoscopy for multi-chromatic probes and for multi-color imaging under low-intensity-level NIR-II CW laser depletion.
PB Nature Research
YR 2022
FD 2022-05-23
LK https://hdl.handle.net/20.500.14352/116613
UL https://hdl.handle.net/20.500.14352/116613
LA eng
NO Natura Communications (2022) 13:2843
DS Docta Complutense
RD 5 abr 2025