RT Journal Article
T1 Immunocytochemical study on the triple origin of the sphincter iris in the chick embryo
A1 Barrio Asensio, María Del Carmen
A1 Murillo González, Jorge Alfonso
A1 Peña Melián, Ángel
A1 Puerta Fonollá, Javier
AB The ontogenic development of the sphincter iris has been studied by immunocytochemistry and standard staining on chick embryos from stage 25 HH to the time of hatching. We have used the monoclonal antibody 13F4, a highly specific marker of muscular cells. We have observed three different regions in the iris. Tn the pupillary region, immunoreactive cells are in continuous contact with the inner epithelium of the pupillary margin. In the intermediate region, the outer epithelium forms buds of pigmented cells that emigrate toward the stroma. In this epithelium cells that are totally or partially unpigmented exist, and they are 13F4 positive. In the sphincter we have observed 13F4 positive cells with melanin granules. In the ciliary region, the immunoreactivity appears in dispersed mesenchymal cells. The present findings are consistent with a triple origin of the sphincter iris in the chick embryo. This muscle is derived from the inner epithelium of the pupillary margin, the intermediate region of the outer epithelium, and from the mesenchymal cells. The cells of the inner epithelium of the pupillary margin are differentiated into smooth muscle cells, and the remaining cells form striated muscle cells.
PB Springer-Verlag
SN 0949-944X
YR 1999
FD 1999-10
LK https://hdl.handle.net/20.500.14352/60010
UL https://hdl.handle.net/20.500.14352/60010
LA eng
NO Barrio Asensio, M. C., Murillo González, J. A., Peña Melián, Á., Puerta Fonollá, J. «Immunocytochemical Study on the Triple Origin of the Sphincter Iris in the Chick Embryo». Development Genes and Evolution, vol. 209, n.o 10, octubre de 1999, pp. 620-24. Springer Link, https://doi.org/10.1007/s004270050296.
DS Docta Complutense
RD 20 jul 2024