RT Journal Article
T1 Aggregation of human polymorphonuclear leukocytes by endothelin: role of platelet-activating factor
A1 Gómez Garre, María Dulce Nombre
A1 Guerra, Manuel
A1 González, Eva
A1 López Farre, Antonio José
A1 Riesco, Amparo
A1 Caramelo, Carlos
A1 Escanero, Jesús
A1 Egido, Jesús
AB The mechanisms by which endothelin-1 (ET-1) acts on polymorphonuclear leukocytes (PMN) are insufficiently known. In this study, we assessed the hypotheses that ET-1 is a PMN-aggregating agent, and that platelet-activating factor (PAF) is the principal mediator of ET-1-induced PMN aggregation. ET-1 induced dose-related PMN aggregation, which started 1 min after ET-1 exposure. Two different specific PAF receptor antagonists blocked the effect of ET-1 on PMN aggregation. In addition, FT-1 induced a significant increase in the production of PAF by PMN after 2 to 5 min of FT-1 incubation. FT 1 induced PAF release from PMN rather than accumulation. This PAF production was dependent on intra- and extracellular Ca2+. In this regard, the PAF receptor antagonists significantly blunted the ET-1-induced peak in cytosolic free Ca2+ ([Ca2+ ]i). Our results, therefore, indicate that ET-1 is effective in causing aggregation of human PMN and that its action appears to be mediated by PAF production via a Ca2+-dependent mechanism.
PB Elsevier
SN 0014-2999
YR 1992
FD 1992
LK https://hdl.handle.net/20.500.14352/117548
UL https://hdl.handle.net/20.500.14352/117548
LA eng
NO Gómez-Garre D, Guerra M, González E, López-Farré A, Riesco A, Caramelo C, Escanero J, Egido J. Aggregation of human polymorphonuclear leukocytes by endothelin: role of platelet-activating factor. Eur J Pharmacol. 1992;224:167-72
NO Instituto de Salud Carlos III (España)
DS Docta Complutense
RD 1 ene 2026