RT Journal Article
T1 Forest cover influences the fecal virome of Oligoryzomys nigripes in Atlantic Forest remnants, Brazil
A1 Sodré, Ingrid Campos
A1 Prist, Paula Ribeiro
A1 Mancini, Matheus Camargo Silva
A1 Bettoni-Rodríguez, Gianmarco
A1 Siliansky De Andreazzi, Cecilia
A1 Reverberi Tambosi, Leandro
A1 Andrade dos Santos, André Felipe
A1 D’arc, Mirela
A1 Bueno, Marina Galvão
AB Landscape changes driven by human activities can alter host-pathogen interactions, favoring generalist mammal species that act as reservoirs for zoonotic pathogens, potentially leading to spillover events and outbreaks. Here, we investigated how forest cover influences viral diversity in Oligoryzomys nigripes, a generalist rodent known to harbor zoonotic viruses in the Brazilian Atlantic Forest. We employed high-throughput sequencing to explore the fecal virome of 20 specimens collected across three landscapes with varying forest cover (20 %, 40 %, and 60 %) within Atlantic Forest fragments in São Paulo state. We identified 48 viral families, predominantly bacteriophages and vertebrate-associated viruses. Some, found for the first time in this host, exhibited zoonotic potential, including Papillomaviridae, Herpesviridae, Polyomaviridae, Adenoviridae, Alloherpesviridae, Arenaviridae, Paramyxoviridae, Peribunyaviridae, and Picornaviridae. Alpha and beta diversity indices were used to assess the viral community structure. Although alpha diversity indices did not show a statistically significant difference among landscapes, a significant compositional difference in viral community was detected through beta diversity index (Jaccard dissimilarity), indicating that forest cover may shape the composition of viral families present. The presence of a core virome shared across all landscapes, including families with pathogenic potential, reinforces O. nigripes role as a natural reservoir. While forest cover influences viral community structure, it doesn't necessarily reflect greater ecological complexity within fragments, indicating that other landscape-related factors must also be considered. This pioneering study characterizes the fecal virome of O. nigripes, revealing how forest cover may shape viral communities in wild rodents and underscoring their potential for zoonotic virus surveillance.
PB Elsevier
SN 0001-706X
YR 2025
FD 2025-10-30
LK https://hdl.handle.net/20.500.14352/134015
UL https://hdl.handle.net/20.500.14352/134015
LA eng
NO Sodré, I. C., Prist, P. R., Mancini, M. C. S., Bettoni-Rodríguez, G., Siliansky de Andreazzi, C., Reverberi Tambosi, L., Andrade dos Santos, A. F., D’arc, M., & Galvão Bueno, M. (2025). Forest cover influences the fecal virome of Oligoryzomys nigripes in Atlantic Forest remnants, Brazil. Acta Tropica, 272, 107894. https://doi.org/10.1016/j.actatropica.2025.107894
NO Funding sourcesThis work was mainly supported by the project Land use change, ecosystem resilience and zoonotic spillover risk (ZOORest) funded by the National Science Foundation (NSF 2225023) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, process 2022/02,174- 5) and partially funded by Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Brazil (FAPERJ; JCNE 200.244/2023) and Instituto Oswaldo Cruz (PAEF-3 and PROEP/CNPQ/2024). MCSM was funded by a post-doctoral grant from FAPESP (2023/10302–6)AcknowledgmentsWe would like to thank the entire ZooRest project team for their assistance with the fieldwork, and The Instituto Nacional do Cancer (INCA) for their support in conducting the high-throughput sequencing (Illumina MiSeq platform).
NO National Science Foundation (Estados Unidos)
NO Fundação de Amparo à Pesquisa do Estado de São Paulo
NO Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
NO Instituto Oswaldo Cruz (Brasil)
DS Docta Complutense
RD 30 mar 2026