%0 Book Section
%T Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay
publisher Humana Press
%D 2025
%U 978-1-0716-4388-4
%U 978-1-0716-4386-0
%U 978-1-0716-4385-3
%@ https://hdl.handle.net/20.500.14352/130741
%X Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine. In this chapter, we describe a procedure of isolation and culture of ovarian cortical cells that consistently allows the spontaneous generation of a large number of neurospheres (NS), and the methods to demonstrate that their integrating NSC/NPC self-renew and are able to differentiate into neurons and glia, during the NS assay.
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