RT Book, Section
T1 Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay
A1 González Gil, Alfredo
A1 Picazo González, Rosa Ana
A2 Ortega De La O, Felipe
AB Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine. In this chapter, we describe a procedure of isolation and culture of ovarian cortical cells that consistently allows the spontaneous generation of a large number of neurospheres (NS), and the methods to demonstrate that their integrating NSC/NPC self-renew and are able to differentiate into neurons and glia, during the NS assay.
PB Humana Press
SN 978-1-0716-4388-4
SN 978-1-0716-4386-0
SN 978-1-0716-4385-3
SN 1064-3745
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/130741
UL https://hdl.handle.net/20.500.14352/130741
LA eng
NO Alfredo González-Gil and Rosa Ana Picazo (2025) Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay. In Methods of Molecular Biology, Neural Stem Cells, Chapter 6. Edited by Felipe Ortega. Ed. Springuer Nature.   https://doi.org/10.1007/978-1-0716-4386-0_6,
NO Ministerio de Economía y Competitividad (España)
NO Universidad Complutense de Madrid
NO Banco Santander
DS Docta Complutense
RD 25 feb 2026