RT Journal Article
T1 Cux-2 Controls the Proliferation of Neuronal Intermediate Precursors of the Cortical Subventricular Zone
A1 Cubelos, Beatriz
A1 Sebastián Serrano, Álvaro
A1 Seonhee, Kim
A1 Moreno-Ortiz, Carmen
A1 Redondo, Juan Miguel
A1 Walsh, Christopher A.
A1 Nieto, Marta
AB Whereas neurons of the lower layers (VI-V) of the cerebral cortex are first born from dividing precursors at the ventricular zone, upper layer neurons (II-IV) subsequently arise from divisions of intermediate neuronal precursors at the subventricular zone (SVZ). Little is known about mechanisms that control the proliferation of SVZ neuronal precursors. We herein report that the restricted expression of the homeodomain transcription factor Cux-2 in the SVZ regulates the proliferation of intermediate neuronal precursors and the number of upper layer neurons. In Cux-2-deficient mice (Cux-2-/-), there is excessive number of upper layer neurons and selective expansion of SVZ neuronal precursors. Double-labeling experiments demonstrate that Cux-2-/- upper layer precursors reenter the cell cycle in a higher frequency than wild-type precursors. Overexpression studies indicate that Cux-2 controls cell cycle exit in a cell-autonomous manner. Analysis of Cux-1-/-; Cux-2-/- double mutant revealed that Cux-2 controls SVZ proliferation independently of Cux-1, demonstrating that this is a unique function of Cux-2, not redundant with Cux-1 activities. Our results point to Cux-2 as a key element in the control of the proliferation rates of the SVZ precursors and the number of upper cortical neurons, without altering the number of deep cortical layers.
PB Oxford University Press
SN 1460-2199
SN 1047-3211
YR 2007
FD 2007
LK https://hdl.handle.net/20.500.14352/96221
UL https://hdl.handle.net/20.500.14352/96221
LA eng
NO Cubelos B, Sebastián-Serrano A, Kim S, Moreno-Ortiz C, Redondo JM, Walsh CA, Nieto M. Cux-2 controls the proliferation of neuronal intermediate precursors of the cortical subventricular zone. Cereb Cortex. 2008 Aug;18(8):1758-70. doi: 10.1093/cercor/bhm199. Epub 2007 Nov 21. PMID: 18033766.
DS Docta Complutense
RD 13 abr 2025