RT Journal Article
T1 Reconstitution of cytomegalovirus-specific T-cell immunity following unmanipulated haploidentical allogeneic hematopoietic stem cell transplantation with posttransplant cyclophosphamide
A1 Huntley, Dixie
A1 Giménez, Estela
A1 Pascual, María Jesús
A1 Remigia, María José
A1 Amat, Paula
A1 Vázquez, Lourdes
A1 Hernández Martín, Marta M.
A1 Hernández Boluda, Juan Carlos
A1 Gago, Beatriz
A1 Piñana, José Luis
A1 García, Magdalena
A1 Martínez, Ariadna
A1 Mateo, Eva
A1 Gozalbo Rovira, Roberto
A1 Albert, Eliseo
A1 Solano, Carlos
A1 Navarro, David
AB AbstractCytomegalovirus (CMV) DNAemia and CMV disease have been reported as more frequent in patients undergoing haploidentical allogeneic hematopoietic stem cell transplantation (Haplo-HSCT) than in those receiving HLA-matched allografts. This could be due to impaired CMV-specific T-cell reconstitution. Here, we conducted a multicenter observationalstudy to assess CMV pp65 and IE-1-specific T cells kinetics in patients undergoing unmanipulated Haplo-HSCT withposttransplant cyclophosphamide (PT/Cy-haplo) and compared it with patients allografted with HLA-matched donors. PlasmaCMV DNA load was monitored by real-time PCR and enumeration of CMV-specific IFN-γ-producing CD8+ and CD4+T cells was performed by flow cytometry for intracellular cytokine staining at days +30, +60, +90, and +180 aftertransplantation. CMV DNAemia developed in 62 patients, occurring with comparable frequency in PT/Cy-haplo and MRD/MUD recipients (P =0.14). There were no significant differences across groups in the number of patients either displayingdetectable CMV-specific CD8+ and CD4+ T-cell responses or acquiring CMV-specific T-cell levels conferring protectionagainst subsequent infection. CMV-specific T-cell counts were comparable between groups at most time points examined,irrespective of whether CMV DNAemia occurred or not prior to monitoring. Collectively the data suggest that PT/Cy-haplorecipients may reconstitute CMV-specific T-cell immunity to the same extent as patients undergoing HLA-matched allo-HSCT.
PB Springer Nature
SN 0268-3369
SN 1476-5365
YR 2020
FD 2020-03-23
LK https://hdl.handle.net/20.500.14352/107122
UL https://hdl.handle.net/20.500.14352/107122
LA eng
NO Huntley, D., Giménez, E., Pascual, M.J. et al. Reconstitution of cytomegalovirus-specific T-cell immunity following unmanipulated haploidentical allogeneic hematopoietic stem cell transplantation with posttransplant cyclophosphamide. Bone Marrow Transplant 55, 1347–1356 (2020). https://doi.org/10.1038/s41409-020-0865-x
DS Docta Complutense
RD 14 may 2026