RT Journal Article
T1 α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
A1 Arias Álvarez, María
A1 García García, Rosa María
A1 López-Tello, Jorge
A1 Rebollar, Pilar
A1 Gutiérrez-Adán, Alfonso
A1 Lorenzo González, Pedro Luis
AB The developmental competence of in vitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding a-tocopherol (0, 100, 200 and 400 mu M) during IVM on putative transcripts involved in antioxidant defence (superoxide dismutase 2, mitochondrial (SOD2), glutathione peroxidase 1 (GPX1), catalase (CAT)), cell cycle regulation and apoptosis cascade (apoptosis tumour protein 53 (TP53), caspase 3, apoptosis-related cysteine protease (CASP3)), cell cycle progression (cellular cycle V-Akt murine thymoma viral oncogene homologue 1 (AKT1)), cumulus expansion (gap junction protein, alpha 1, 43 kDa (GJA1) and prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclo-oxygenase) (PTGS2)) and metabolism (glucose-6-phosphate dehydrogenase (G6PD)). Meiotic progression, mitochondrial reallocation, cumulus cell apoptosis and the developmental competence of oocytes after IVF were also assessed. Expression of SOD2, CAT, TP53, CASP3 and GJA1 was downregulated in cumulus-oocyte complexes (COCs) after IVM with 100 mu M alpha-tocopherol compared with the group without the antioxidant. The apoptotic rate and the percentage of a non-migrated mitochondrial pattern were lower in COCs cultured with 100 mu M alpha-tocopherol, consistent with better-quality oocytes. In fact, early embryo development was improved when 100 mu M alpha-tocopherol was included in the IVM medium, but remained low compared with in vivomatured oocytes. In conclusion, the addition of 100 mu M alpha-tocopherol to the maturation medium is a suitable approach to manage oxidative stress and apoptosis, as well as for increasing the in vitro developmental competence of rabbit oocytes.
PB CSIRO Publishing
SN 1031-3613
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/93700
UL https://hdl.handle.net/20.500.14352/93700
LA eng
NO Arias-Álvarez, M., et al. «α-Tocopherol Modifies the Expression of Genes Related to Oxidative Stress and Apoptosis during in Vitro Maturation and Enhances the Developmental Competence of Rabbit Oocytes». Reproduction, Fertility and Development, vol. 30, n.o 12, 2018, p. 1728. https://doi.org/10.1071/RD17525.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid 
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 10 abr 2025