RT Journal Article T1 Changes induced by pressure processing on immunoreactive proteins of tree nuts A1 Cuadrado Hoyos, Carmen A1 Sanchiz, África A1 Vicente, Fátima A1 Ballesteros Redondo, María Isabel A1 Linacero De La Fuente, M. Rosario AB Tree nuts confer many health benefits due to their high content of vitamins and antioxidants, and they are increasingly consumed in the last few years. Food processing is an important industrial tool to modify allergenic properties of foods, in addition to ensuring safety and enhancing organoleptic characteristics. The effect of high pressure, without and with heating, on SDS-PAGE and immunodetection profile of potential allergenic proteins (anti-11S, anti-2S and anti-LTP) of pistachio, cashew, peanut, hazelnut, almond, and chestnut was investigated. Processing based on heat and/or pressure and ultra-high pressure (HHP, 300–600 MPa) without heating was applied. After treating the six tree nuts with pressure combined with heat, a progressive diminution of proteins with potential allergenic properties was observed. Moreover, some tree nuts proteins (pistachio, cashew, and peanut) seemed to be more resistant to technological processing than others (hazelnut and chestnut). High pressure combined with heating processing markedly reduce tree nut allergenic potential as the pressure and treatment time increases. HHP do not alter hazelnut and almond immunoreactivity. PB MDPI SN 1420-3049 YR 2020 FD 2020 LK https://hdl.handle.net/20.500.14352/6181 UL https://hdl.handle.net/20.500.14352/6181 LA eng NO Cuadrado, C.; Sanchiz, A.; Vicente, F.; Ballesteros, I.; Linacero, R. Changes Induced by Pressure Processing on Immunoreactive Proteins of Tree Nuts. Molecules 2020, 25, 954. https://doi.org/10.3390/molecules25040954 NO Ministerio de Ciencia e Innovación (España) DS Docta Complutense RD 8 abr 2025