RT Journal Article
T1 Polyphenols and IUGR Pregnancies: Intrauterine Growth Restriction and Hydroxytyrosol Affect the Development and Neurotransmitter Profile of the Hippocampus in a Pig Model
A1 Yeste, Natalia
A1 Gómez, Néstor
A1 Vázquez Gómez, Marta
A1 Garcia Contreras, Consolación
A1 Pumarola, Martí
A1 González Bulnes, Antonio
A1 Bassols, Anna
AB Intrauterine growth restriction (IUGR) refers to poor growth of a fetus during pregnancy due to deficient maternal nutrition or oxygen supply. Supplementation of a mother’s diet with antioxidants, such as hydroxytyrosol (HTX), has been proposed to ameliorate the adverse phenotypes of IUGR. In the present study, sows were treated daily with or without 1.5 mg of HTX per kilogram of feed from day 35 of pregnancy (at 30% of the total gestational period), and fetuses were sampled at day 100 of gestation. Fetuses were classified as normal body weight (NBW) or low body weight (LBW) as a consequence of IUGR, constituting four groups: NBW-Control, NBW-HTX, LBW-Control, and LBW-HTX. The brain was removed, and the hippocampus, amygdala, and prefrontal cortex were rapidly dissected. Neuronal markers were studied by immunohistochemistry, and a decrease in the number of mature neurons in the hippocampal Cornu Ammonis subfield 1 (CA1) and the Dentate Gyrus (DG) regions was observed in LBW fetuses together with a higher number of immature neurons and other alterations in neuronal morphology. Furthermore, IUGR conditions altered the neurotransmitter (NT) profile, since an increase in the serotonin (5-HT) pathway was observed in LBW fetuses. Supplementation with HTX was able to reverse the morphological and neurochemical changes, leading both characteristics to values similar to those of NBW fetuses.
PB MPDI
SN 2076-3921
YR 2021
FD 2021-09-22
LK https://hdl.handle.net/20.500.14352/5028
UL https://hdl.handle.net/20.500.14352/5028
LA eng
NO Spanish Ministry of Economy and Competitiveness/FEDER
NO Ministry of Science, Innovation and Universities
NO FPI National Program Grant
DS Docta Complutense
RD 20 abr 2025